Toxicity of Five Root Canal Sealers in vitro

Aim of the study was to evaluate cytotoxicity of five root canal sealers at different intervals of time on HEK-293 (human embryonic kidney epithelial cells) and Vero cells (African green monkey kidney epithelial cells). Endomethasone N, EndoREZ, Acroseal, RoekoSeal and AH Plus were mixed according to manufacturer’s instructions. MTT assay was used to evaluate cytotoxicity of freshly mixed and set sealers 0 h, 24 h, 48 h, 72 h and 1week on target cells differently. Statistical analysis was done using SPSS Version 15.0 (SPSS, Inc., Chicago, IL, USA) statistical software package (p < 0.05). The study concluded that cytotoxicity of freshly mixed sealers start increasing and reaches to maximum at 24-48hr, then starts decreasing and reaches to a stable level at a week interval. Endomethasone N exhibit highest toxicity while EndoREZ was least toxic towards cultured cells. Acroseal, RoekoSEAL and AH-Plus showed intermediate cytotoxicity.